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Autophagy:华南农大张炼辉课题组发表病原真菌致病机制研究成果

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摘要 : 2017年6月8日,国际著名自噬领域学术期刊《Autophagy》在线发表在线发表了华南农业大学农学院张炼辉研究组题为“Phototrophy and starvation-based induction of autophagy upon removal of Gcn5-catalyzed acetylation of Atg7 in Magnaporthe oryzae”的研究论文
2017年6月8日,国际著名自噬领域学术期刊《Autophagy》在线发表在线发表了华南农业大学农学院张炼辉研究组题为“Phototrophy and starvation-based induction of autophagy upon removal of Gcn5-catalyzed acetylation of Atg7 in Magnaporthe oryzae”的研究论文,研究揭示了植物病原真菌稻瘟病菌感知光照信号而诱导自噬与自身致病性分化-分生孢子产生的细胞生物学机制。博士生张树林与梁美玲为共同第一作者,张炼辉教授与邓懿祯教授为共同通讯作者。 稻瘟病菌可以响应光照而诱导分生孢子的产生,且这一过程依赖自噬这一真核生物保守的大分子降解途径。邓懿祯教授早期的研究结果揭示了自噬主要参与稻瘟病菌细胞质糖原储备的降解,为分生孢子产生提供能量或结构材料,但自噬响应光照信号诱导的信号通路尚未解析。继2015年该团队成员在国际著名期刊PLOS Pathogens (SCI生物学1区,IF2015=7.003)发表稻瘟病菌的光照响应机制研究论文“Twilight, a novel circadian-regulated gene, integrates phototropism with nutrient and redox homeostasis during fungal development”之后,此论文进一步鉴定出一个在转录与蛋白亚细胞定位水平响应光照调控的组蛋白乙酰转移酶Gcn5,通过直接修饰自噬蛋白Atg7而实现对自噬诱导的调控,从而揭示了稻瘟病菌所特有的光照诱导自噬现象的分子机制。稻瘟病菌Gcn5对自噬的调控与其植物或动物同源基因/蛋白调控自噬的模式有所差异,因此有可能作为稻瘟病害防控的一个潜在靶点。 原文链接: Phototrophy and starvation-based induction of autophagy upon removal of Gcn5-catalyzed Acetylation of Atg7 in Magnaporthe oryzae 原文摘要: Magnaporthe oryzae, the ascomycete fungus that causes rice blast disease, initiates conidiation in response to light when grown on Prune-Agar medium containing both carbon and nitrogen sources. Macroautophagy/autophagy was shown to be essential for M. oryzae conidiation and induced specifically upon exposure to light but is undetectable in the dark. Therefore, it is inferred that autophagy is naturally induced by light, rather than by starvation during M. oryzae conidiation. However, the signaling pathway(s) involved in such phototropic induction of autophagy remains unknown. We identified an M. oryzae ortholog of GCN5 (MGG_03677), encoding a histone acetyltransferase (HAT) that negatively regulates light- and nitrogen-starvation-induced autophagy, by acetylating the autophagy protein Atg7. Furthermore, we unveiled novel regulatory mechanisms on Gcn5 at both transcriptional and post-translational levels, governing its function associated with the unique phototropic response of autophagy in this pathogenic fungus. Thus, our study depicts a signaling network and regulatory mechanism underlying the autophagy induction by important environmental clues such as light and nutrients. DOI:10.1080/15548627.2017.1327103 作者:张炼辉 点击:
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