当前位置:生物帮 > 期刊文章 > PNAS > 正文

PNAS:利用斑马鱼找到尼古丁成瘾的秘密

摘要 : 科学家开发出了一种寻找可能造成改变生物体对尼古丁的生理与行为响应的遗传变异的系统。研究表明,个体对于尼古丁(烟草的成瘾成分)的响应严重取决于它们的遗传结构。 Stephen Ekker及其同事使用一种称为斑马鱼的模式生物,并把动物的已知能改变对于成瘾以及肺癌易发性体质的遗传变异引入到它的身上。这组科学家在水中加入了各种浓度的尼古丁,然后监测了每一条转基
科学家开发出了一种寻找可能造成改变生物体对尼古丁的生理与行为响应的遗传变异的系统。研究表明,个体对于尼古丁(烟草的成瘾成分)的响应严重取决于它们的遗传结构 Stephen Ekker及其同事使用一种称为斑马鱼模式生物,并把动物的已知能改变对于成瘾以及肺癌易发性体质的遗传变异引入到它的身上。这组科学家在水中加入了各种浓度的尼古丁,然后监测了每一条转基因鱼的活动,从而测试这些鱼的尼古丁响应。当这些鱼接触到高浓度的尼古丁的时候,它们的运动就减慢了。对于中间剂量,这些鱼游动得更快。这种遗传筛查系统还探测到了两种此前未被发现的存在于人类身上的遗传变异类型,它们也影响斑马鱼的尼古丁响应。这组作者说,这项研究可能提供了证据表明斑马鱼是研究成瘾的一种合适的模型,而且可能用于发现让人们倾向于各种类型的成瘾的遗传标记。 原文摘要: Nicotine response genetics in the zebrafish Tobacco use is predicted to result in over 1 billion deaths worldwide by the end of the 21st century. How genetic variation contributes to the observed differential predisposition in the human population to drug dependence is unknown. The zebrafish (Danio rerio) is an emerging vertebrate model system for understanding the genetics of behavior. We developed a nicotine behavioral assay in zebrafish and applied it in a forward genetic screen using gene-breaking transposon mutagenesis. We used this method to molecularly characterize bdav/cct8 and hbog/gabbr1.2 as mutations with altered nicotine response. Each have a single human ortholog, identifying two points for potential scientific, diagnostic, and drug development for nicotine biology and cessation therapeutics. We show this insertional method generates mutant alleles that are reversible through Cre-mediated recombination, representing a conditional mutation system for the zebrafish. The combination of this reporter-tagged insertional mutagen approach and zebrafish provides a powerful platform for a rich array of questions amenable to genetic-based scientific inquiry, including the basis of behavior, epigenetics, plasticity, stress, memory, and learning. 作者:网友 点击:
    热门文章TOP
    优发国际